Purushothama Rao Tata, Ph.D. (University of Ulm, Ulm, Germany)
Assistant Professor, Department of Cell Biology
308 Nanaline Duke Bldg., Box 3709
Duke University School of Medicine
Durham, NC 27710
Genetic and epigenetic basis of cell identity and plasticity in regeneration and tumorigenesis
Many human diseases are a result of either a deficit or surfeit in the quantity or functionality of particular cell types. Although the principles of cell fate specification during embryogenesis are rather well documented, it is unclear what mechanisms are used to maintain cell identity and appropriate cell numbers in adult tissues. While the cell autonomous molecular processes defining cell states in both physiological and pathological conditions are being defined with increasing precision using genome-wide inventories, the question of when and how individual cell types interact with one another to maintain their identity remains largely unresolved. In this context Tata lab studies:
i) Defining the cellular heterogeneity and the lineage hierarchies in epithelial tissues
ii) How do individual cells maintain their identity under steady state conditions and in the context of regeneration?
ii) The genetic and epigenetic basis of tissue plasticity associated with regeneration and tumorigenesis
Tata lab focuses on understanding the ensemble properties of tissues in the context of development, homeostasis, regeneration and tumorigenesis in diverse epithelial tissues including lung. We use mouse models of disease that recapitulates the human disease pathology. We utilize genetic, live imaging, cell biological and next generation sequencing technologies including single-cell RNA sequencing to study the behavior of tissues at single cell level.
Tata PR, Rajagopal J. (2017) Plasticity in the lung: making and breaking cell identity. Development. 1;144(5):755-766.
Saladi SV, Ross K, Karaayvaz M, Tata PR, Mou H, Rajagopal J, Ramaswamy S, Ellisen LW. (2017) ACTL6A Is Co-Amplified with p63 in Squamous Cell Carcinoma to Drive YAP Activation, Regenerative Proliferation, and Poor Prognosis. Cancer Cell. 9;31(1):35-49.
Tata PR, Rajagopal J. (2016) Regulatory Circuits and Bi-directional Signaling between Stem Cells and Their Progeny. Cell Stem Cell. 1;19(6):686-689.
Tata PR, Rajagopal J. (2016) Cellular plasticity: 1712 to the present day. Curr Opin Cell Biol. 43:46-54. Review.
Mou H, Vinarsky V, Tata PR, Brazauskas K, Choi SH, Crooke AK, Zhang B, Solomon GM, Turner B, Bihler H, Harrington J, Lapey A, Channick C, Keyes C, Freund A, Artandi S, Mense M, Rowe S, Engelhardt JF, Hsu YC, Rajagopal J. (2016) Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells. Cell Stem Cell. 4;19(2):217-31.
Pardo-Saganta A, Tata PR, Law BM, Saez B, Chow RDz, Prabhu M, Gridley T, Rajagopal J. (2015) Parent stem cells can serve as niches for their daughter cells. Nature. 30;523(7562):597-601.
Pardo-Saganta A, Law BM, Tata PR, Villoria J, Saez B, Mou H, Zhao R, Rajagopal J. (2015) Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations. Cell Stem Cell. 5;16(2):184-97.
Weidgang CE, Russell R, Tata PR, Kühl SJ, Illing A, Müller M, Lin Q, Brunner C, Boeckers TM, Bauer K, Kartikasari AE, Guo Y, Radenz M, Bernemann C, Weiß M, Seufferlein T, Zenke M, Iacovino M, Kyba M, Schöler HR, Kühl M, Liebau S, Kleger A. (2014) TBX3 Directs Cell-Fate Decision toward Mesendoderm. Stem Cell Reports. 6;2(5):747.
Weidgang CE, Russell R, Tata PR, Kühl SJ, Illing A, Müller M, Lin Q, BrunnerC, Boeckers TM, Bauer K, Kartikasari AE, Guo Y, Radenz M, Bernemann C, Weiß M, Seufferlein T, Zenke M, Iacovino M, Kyba M, Schöler HR, Kühl M, Liebau S, Kleger A. (2013) TBX3 Directs Cell-Fate Decision toward Mesendoderm. Stem Cell Reports. 29;1(3):248-65.
Tata PR, Mou H, Pardo-Saganta A, Zhao R, Prabhu M, Law BM, Vinarsky V, Cho JL, Breton S, Sahay A, Medoff BD, Rajagopal J. (2013) Dedifferentiation of committed epithelial cells into stem cells in vivo. Nature. 14;503(7475):218-23.