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Blanche Capel, Ph.D.
(Genetics, University of Pennsylvania)


Professor,
Department of Cell Biology

Duke University Appointments and Tenure Committee
Director of Admissions, Developmental Biology Training Program
Sex Determination, Testis and Ovary Development, and Germ Cell Biology
   The process of sex determination in mammals is dependent on the gonadal expression of a single gene on the Y chromosome, Sry. However, downstream of this dominant genetic switch are complex antagonistic signaling pathways that wage the battle of the sexes in the bipotential gonad. These signals determine the fate of gonadal cells and lead to the development of either a testis or an ovary. We study the cell and molecular pathways that regulate organogenesis of the testis and ovary. Other work in the lab centers on a comparison to temperature dependent sex determination in the red eared slider turtle Trachemys scripta elegans, and the biology of germ cells in the testis and ovary. Experimental approaches include organ culture, transgenic mice, confocal microscopy and live imaging, biochemical and molecular techniques, comparative embryology, classic mouse genetics, and systems biology.



E-mail b.capel@cellbio.duke.edu

4026 GSRBII Bldg., Box 3471
Duke University Medical Center
Durham, NC 27710

Telephone 919-684-6390
Fax 919-668-3467

Selected publications
Samantha A. Jameson and Blanche Capel. Testis development requires the repression of Wnt4 by Fgf sginaling. Dev. Biol. 370(1):24-32 (2012). PMID:22705479 [PMC journal – in process] -PDF-

Munger, SC and Capel, B. Sex and the circuitry: progress toward a systems-level understanding of vertebrate sex determination. Wiley Interdiscip Rev Syst Biol Med (epub 2012). PMID:22605638. -PDF-

Mork, L, Maatouk, DM, McMahon, JA, Guo, JJ, Zhang, P, McMahon, AP, and Capel, B. Temporal differences in granulosa cell specification in the ovary reflect distinct follicle fates. BiolReprod 86:37 (2012). PMID: 21976597. PMC3290667. -PDF-

Samantha Jameson, Anirudh Natarajan, Jonah Cool, Tony De Falco, Danielle Maatouk, Lindsey Mørk, Steve Munger, and Blanche Capel. Temporal transcriptional profiling of somatic and germ cells reveals lineage priming of sexual fate in the fetal mouse gonad. PLoS Genetics 8:e1002575 (2012). PMID: 22438826. PMC3305395. -PDF-

Lindsey Mork, Hao Tang, and Blanche Capel. Mouse germ cell clusters form by aggregation was well as clonal divisions. MechDev 128:591-596 (2012). PMID:22245112. PMC3295915. -PDF-

Maatouk DM, Mork L, Hinson A, Kobayashi A, McMahon AP, Capel B. Germ cells are not required to establish the female pathway in mouse fetal gonads. PLoS One. 7(10):e47238 (2012). PMID: 23091613. -PDF-

Cool J, DeFalco TJ, Capel B. Vascular-mesenchymal cross-talk through Vegf and Pdgf drives organ patterning. Proc Natl Acad Sci U S A. 108(1):167-72.(2011). PMID: 21173261 PMC3017142. -PDF-

Cook MS, Munger SC, Nadeau JH, and Capel B... Regulation of male germ cell cycle arrest and differentiation by DND1 is modulated by genetic background. Development 138:23-32 (2011). PMID: 21115610. PMC2998163. -PDF-

DeFalco T, Takahashi S, Capel B. Two distinct origins for Leydig cell progenitors in the fetal testis. Dev Biol. 352(1):14-26 (2011). PMID: 21255566 PMC3055913. -PDF-

Barske LA, Capel B. Estrogen represses SOX9 during sex determination in the red-eared slider turtle Trachemys scripta. Dev Biol. 341(1):305-14 (2010). PMID: 20153744 PMC2930626. -PDF-

Munger SC, Aylor DL, Syed HA, Magwene PM, Threadgill DW, Capel B. Elucidation of the transcription network governing mammalian sex determination by exploiting strain-specific susceptibility to sex reversal. Genes Dev. 23(21):2521-36 (2009). PMID: 19884258 PMC2779749. -PDF-

Barske LA and Capel B. Blurring the edges in vertebrate sex determination. Curr Opin Genet Dev. 18:499-505 (2009). PMID: 19152784 PMC2660668. -PDF-

Cook M, Coveney D, Batchvarov I, Joe H. Nadeau JH, Capel B. BAX-mediated cell death affects early germ cell loss and incidence of testicular teratomas in Dnd1(Ter/Ter) mice. DevBiol 328:377-383 (2009). PMID: 19389346. PMC2689365. -PDF-

Tang H, Brennan J, Karl J, Hamada Y, Raetzman L, Capel B. Notch signaling maintains mesenchymal progenitor cells in the mouse testis. Development 135:3745-3753 (2008). PMID: 18927153. -PDF-

Current projects
  • Cellular interactions during testis and ovary organogenesis
  • Recovery of the ovary after chemotherapy
  • Mechanism of temperature dependent sex determination
  • Evolution of sex-determining mechanisms
  • Regulation of germ cells by the RNA-binding protein DND1
  • Germ cell tumors/testicular teratomas

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