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Disclaimer: These sequences have been collected over the years and
added to the alignment, always by hand. I have not gone back and
checked for accuracy, so there may be mistakes. Sorry, I haven't even
tabulated all the accession numbers, but I will state that the best
reference for human TN-C is X78565 (Ghezi...Zardi JBC
270:3429-3434), which has corrected a few errors in previous
entries.
Intron-exon boundaries have been determined for TNChum, TNRhum,
TNXhum, and partially
for TNXpig. These are indicated by Red and double underline, but only
in a separate WORD file.
;
First 22 aa missing from cell culture hum TN-C (Zardi)
TNRhum M G A D GET V V L K N M L I G V N L I L L G
TNRrat M G I E GET V V L K N M L I G V N L I L L G
TNRchk M G T D SEN P V L R N V L I S F N L L L L G
TNCchk M G L P S Q V L A C A I L G L L Y Q H A S G
TNChum M G A M T Q L L A G V F L A F L A L A T E G
mouse M G A V T W L L P G I F L A L F A L T P E G
pig M G V V T R L L V G T F L A S L A L P A Q G
nwt M E A T GCARV L A C L T L R L L C H H S D G
These aa also missing from chick brain TN-C (tissue TN-C?; Jones)
TNRhum S M I K P S E C Q L EVTTERVQRQSVEE
TNRrat S M L K P S E C R L EVTTERVQRQTVEE
TNRchk A V L K P F E C R L EVTTEPAERPAVDE
TNCchk G L I K R I I R Q K R
TNChum G V L K K V I R H K R
mouse G V L K K I I R H K R
pig G V L K K V I R H K R
nwt G L I K K I I R Q K R
TNRhum E G G IAN Y N T S S K E Q P V V F N H V Y N I N V P L D N L C S S G L E A S A EQE
TNRr E G G ASS Y N T S S K E Q P M V F N H V Y N I N V P L E S L C S S G L E A S A EQD
TNRc E G G LAN C S P P V K E Q P M V F H H I Y N I N V P V D S C C S S M L R S S A E E
TNCchk E T G L N V T L P E D N Q P V V F N H V Y N I K L P V G S L C S V D L D T A S G D
TNChum Q S G V N A T L P E E N Q P V V F N H V Y N I K L P V G S Q C S V D L E S A S G E
m E S G L N M T L P E E N Q P V V F N H I Y N I K L P M G S Q C S V D L E S A S G E
p Q T G V N V T L P E E S Q P V V F N H V Y N I K L P V G S Q C S V D L E S A S G D
nwt E S GLTSN V T L P D D N Q P V V F N H V Y N I N L P M G S M C S V D L D P V P G G
Underlined segment in TNRchk is alternatively spliced! EPPVLASE\
TNXhum .....no homology, no Cys....G E K Q V V F T H R I N L PPS T G C G C P P G T ^
TNRhum V S . . A E D E T L A E Y M G Q T S D H E S Q V T F T H R I N F P K K A C P C A S SAQ V
TNRrat V S . . A E D D T L A E Y T G Q T S D H E S Q V T F T H K I N L P K K A C P C A S SAQ V
TNRchk V S . . S E D D R L A E Y T E Q T S D S E S Q V T F T H R I N L P K Q A C K C S T SLP S
TNCchk A D L K A E I E P V K N Y E E H T V N E G N Q I V F T H R I N I P R R A C G C A A A P D
TNChum K D L A P P S E P S E S F Q E H T V D G E N Q I V F T H R I N I P R R A C G C A A A P D
mouse K D L T P T P E S S G S F Q E H T V D G E N Q I V F T H R I N I P R R A C G C A A A P D
pig K D L A A P S E P S E S V Q E H T V D G E N Q I V F T H R I N I P R R A C G C A A A P D
nwt A G Q N A L S E P G S Q Q E E H T V D G N N Q I V F T H R I N I R . G A C G C A A A P D
heptad of
alpha-helix * * * * * * *
TNXhum V Q A L R V R L E I L E E L V K G L K E Q C T . G . G C C
TNRhum L Q E L L S R I E M L E R E V S V L R D Q C . . N A N C C
TNRrat L Q E L L S R I E M L E R E V S V L R D Q C . . N T N C C
TNRch L Q E L L S R I E M L E R E V S M L R D Q C . . N S N C C
TNCchk I K D L L S R L E E L E G L V S S L R E Q C A S G A G C C
TNChum V K E L L S R L E E L E N L V S S L R E Q C T A G A G C C
mouse V K E L L S R L E E L E L L V S S L R E Q C T M G T G C C
pig V K E L L S R L E E L E N L V S S L R E Q C T S G A G C C
nwt I K Y L L S L L E E L E G L V S S L R E Q C T S G A G C C
TNXhum PAS A Q A G T G Q T D V R T L C S L H G V F D L S R C T
TNRhum . Q E S A A T G Q L D Y I P H C S G H G N F S F E S C G
TNRrat . Q E S A A T G Q L D Y V P H C S G H G N F S F E S C G
TNRchk . Q E N A A T G R L D Y T L P C S G H G N F S L E S C R
TNCchk P N S Q T A E G R L D T A P Y C S G H G N Y S T E I C G
TNChum L . . Q P A T G R L D T R P F C S G R G N F S T E G C G
mouse L . . Q P A E G R L D T R P F C S G R G N F S A E G C G
pig L . . Q P A E G R L D T R P F C S G R G N F S T E G C G
nwt G G S Q T A E G A V D T K P Y C N G R G N Y S T E T C S
EGF-like domains TNXhum C S C E P G W G G P T C TNRhum C I C N E G W F G K N C TNRrat C I C N E G W F G K N C TNRchk (it fits as well here as any) C I C S E G W A G S N C TNCchk C V C E P G W K G P N C TNChum C V C E P G W K G P N C m C V C E P G W K G P N C p C V C E P G W K G P N C n C I C E P G W T G P N C TNRegf domains put at the end. No attempt was made to align TNXegf domains EGF domain number 1 TNCchk S E P A C P R N C L N R G L C V R G K C I C E E G F T G E D C TNChum S E P E C P G N C H L R G R C I D G Q C I C D D G F T G E D C m S E P D C P G N C N L R G Q C L E G Q C I C D E G F T G E D C p S E P E C P S N C H L R G Q C V D G Q C V C N E G F T G E D C n S E S E C P G H C H N R G K C V N G K C I C D E G F T G M D C EGF #2 G is missing from Zardi seq TNCchk S Q A A C P S D C N D Q G K C V D G V C V C F E G Y T G P D C TNChum S Q L A C P S D C N D Q G K C V N G V C I C F E G Y A G A D C m S Q L A C P N D C N D Q G R C V N G V C V C F E G Y A G P D C p S Q L A C P S D C N D Q G K C V N G V C V C F E G Y S G V D C n S E V T C P D D C N D Q G R C V N G I C V C F E G Y G G E D C EGF #3 TNCchk G E E L C P H G C G I H G R C V G G R C V C H E G F T G E D C TNChum S R E I C P V P C S EEH G T C V D G L C V C H D G F A G D D C m G L E V C P V P C S EEH G M C V D G R C V C K D G F A G E D C p S R E T C P V P C S EEH G R C V D G R C V C Q E G F A G E D C n G Q E I C Q V E C S E F G K C V N G Q C V C D E G F T G E D C EGF #4 very conserved TNCchk N E P L C P N N C H N R G R C V D N E C V C D E G Y T G E D C TNChum N K P L C L N N C Y N R G R C V E N E C V C D E G F T G E D C m N E P L C L N N C Y N R G R C V E N E C V C D E G F T G E D C p N E P L C L H N C H G R G R C V E N E C V C D E G F T G E D C n S E P R C P N N C N N R G R C V E D E C V C D E G F T G D D C EGF #5 very conserved TNCchk G E L I C P N D C F D R G R C I N G T C F C E E G Y T G E D C TNChum S E L I C P N D C F D R G R C I N G T C Y C E E G F T G E D C m S E L I C P N S C F D R G R C I N G T C Y C E E G F T G E D C p G E L I C P K D C F D R G R C I N G T C Y C D E G F E G E D C n S E L I C P N D C F D R G R C I N G V C F C D E G F T G E D C EGF #6 is poorly conserved, especially on left TNCchk G E L T C P N N C N G N G R C E N G L C V C H E G F V G D D C TNChum G K P T C P H A C H T Q G R C E E G Q C V C D E G F A G L D C m G E L T C P N D C Q G R G Q C E E G Q C V C N E G F A G A D C p G R L A C P H G C R G R G R C E E G Q C V C D E G F A G A D C n G E L T C P N N C N N R G R C V N G L C V C D D G F Q G D D C chicken #7 not very similar TNCchk S Q K R C P K D C N N R G H C V D G R C V C H E G Y L G E D C TNChum S E K R C P A D C H N R G R C V D G R C E C D D G F T G A D C m S E K R C P A D C H H R G R C L N G Q C E C D D G F T G A D C p S E R R C P S D C H N R G R C L D G R C E C D D G F E G E D C n S E L R C P N D C N D R G R C V N G K C V C K E G F M G E D C chicken #8 is missing TNChum G E L K C P N G C S G H G R C V N G Q C V C D E G Y T G E D C m G D L Q C P N G C S G H G R C V N G Q C V C D E G Y T G E D C p G E L R C P G G C S G H G R C V N G Q C V C D E G R T G E D C EGF #9 TNCchk G E L R C P N D C H N R G R C I N G Q C V C D E G F I G E D C TNChum S Q L R C P N D C H S R G R C V E G K C V C E Q G F K G Y D C m S Q R R C P N D C H N R G L C V Q G K C I C E Q G F K G F D C p S Q L R C P N D C H G R G R C V Q G R C E C E H G F Q G Y D C n A D L R C P N D C N N R G R C V N G Q C V C D E G F M G E D C EGF #10 TNCchk G E L R C P N D C H N R G R C V N G Q C E C H E G F I G E D C TNChum S D M S C P N D C H Q H G R C V N G M C V C D D G Y T G E D C m S E M S C P N D C H Q H G R C V N G M C I C D D D Y T G E D C p S E M S C P H D C H Q H G R C V N G M C V C D D G Y T G E D C n S D L R C P G D C N N R G R C V N G Q C V C D E G F R G E D C EGF #11 TNRhum S E P Y C P L G C S S R G V C V D G Q C I C D S E Y S G D D C TNRrat S E P Y C P L G C S S R G V C V D G Q C I C D S E Y S G D D C TNRchk S E P R C P R G C S S R G V C L E G Q C V C D N D Y G G E D C TNCchk G E L R C P N D C N S H G R C V N G Q C V C D E G Y T G E D C TNChum R D R Q C P R D C S N R G L C V D G Q C V C E D G F T G P D C m R D R R C P R D C S Q R G R C V D G Q C I C E D G F T G P D C p R E L R C P G D C S Q R G R C V D G R C V C E H G F A G P D C n G E L R C P D D C N N R G V C V N G Q C I C D E G F M G E N C EGF #12 TNRhum S E L R C P T D C S S R G L C V D G E C V C E E P Y T G E D C TNRrat S E L R C P T D C S S R G L C V D G E C V C E E P Y T G E D C TNRchk S Q L R C P A G C G S R G L C V D G E C I C E E G F G G E D C TNCchk G E L R C P N D C H N R G R C V E G R C V C D N G F M G E D C TNChum A E L S C P N D C H G Q G R C V N G Q C V C H E G F M G K D C m A E L S C P S D C H G H G R C V N G Q C I C H E G F T G K D C p A D L A C P S D C H G R G R C V N G Q C V C H E G F T G K D C n G E L R C P N D C K N R G R C V N G Q C I C D D G F K G E D C EGF #13 TNRhum R E L R C P G D C S G K G R C A N G T C L C E E G Y V G E D C TNRrat R E L R C P G D C S G K G Q C A N G T C L C Q E G Y A G E D C TNRchk S Q P R C P R D C S G R G H C D N G T C V C A E G Y A G E D C TNCchk G E L S C P N D C H Q H G R C V D G R C V C H E G F T G E D C TNChum K E Q R C P S D C H G Q G R C V D G Q C I C H E G F T G L D C m K E Q R C P S D C H G Q G R C E D G Q C I C H E G F T G L D C p G Q R R C P G D C H G Q G R C V D G Q C V C H E G F T G L D C n S E L R C P D D C N D R G R C I N G Q C V C A E G F T G E N C EGF # 14. this TNR seq is a miserable fit. TNRhum G Q R Q C L N A C S G R G Q C E E G L C V C E E G Y Q G P D C TNRrat S Q R R C L N A C S G R G H C Q E G L C I C E E G Y Q G P D C TNRchk G W L R C P N A C S G R G V C Q D G L C I C E D G Y G G Q D C TNCchk R E R S C P N D C N N V G R C V E G R C V C E E G Y M G I D C TNChum G Q H S C P S D C N N L G Q C V S G R C I C N E G Y S G E D C m G Q R S C P N D C S N Q G Q C V S G R C I C N E G Y T G I D C p G Q R S C P N D C S N W G Q C V S G R C I C N E G Y S G E D C n D S L A C L N N C N D R G L C V N G Q C V C E E G F L G E D C FN-III domains- HxB
HxB FN-III domain #1
TNRhum SAVAPPEDLRVAGISDRSIELEWDGPMAVTE.YVISYQPTALGGLQLQQRVPG..DWSGVTITELEPGLTYNISVYAVISNILSLPITAKVAT
TNRrat SAVTPPEDLRVAGISDRSIELEWDGPMAVTE.YVISYQPSL.GGLQLQQRVPG..DWSGVTITELEPGLTYNISVYAVISNILSLPITAKVAT
TNRchk SAVAPPENLRVTGISDGSIELAWDSLGAATE.YVVSYQPAGPGGSQLQQRVPG..DWSTITITELEPGVAYNVSIYAVISDVLSSPVTTKVTT
TNCchk SDVSPPTELTVTNVTDKTVNLEWKHENLVNE.YLVTYVPTSSGGLDLQFTVPG..NQTSATIHELEPGVEYFIRVFAILKNKKSIPVSARVAT
TNChum SEVSPPKDLVVTEVTEETVNLAWDNEMRVTE.YLVVYTPTHEGGLEMQFRVPG..DQTSTIIQELEPGVEYFIRVFAILENKKSIPVSARVAT
m SEVSPPKDLIVTEVTEETVNLAWDNEMRVTE.YLIMYTPTHADGLEMQFRVPG..DQTSTTIRELEPGVEYFIRVFAILENKRSIPVSARVAT
p SQVSPPKDLIVTEVTEETVNLAWDNEMRVTE.YLIVYTPTHEDGLEMQFRVPG..DQTSTTIRELEPGVEYFIRVFAILENKKSIPVSARVAT
n SEVSPPKDLTVTDVTTQSVNLEWANEMKVTE.YLITYIPTSPGGLELDFRVPG..DQTTATIQELEPGVEYFVRVFAILRNQRSIPVSARVAT
HxB FN-III domain #2
TNRhum .HLSTPQGLQFKTITETTVEVQWEPFSFSFDGWEISFIPKNN.E..GGVIAQVPSDVTSFNQTGLKPGEEYIVNVV.ALKEQARSPPTSASVST
TNRrat HLSTPQGLQFKTITETTVEVQWEPFSFSFDGWEISFTPKNN.E..GGVIAQLPSDVTSFNQTGLKPGEEYIVNVV.ALKEQARGPPTSASVST
TNRchk NLATPQGLKFKTITETTVEVQWEPFSFPFDGWEISFIPKNN.E..GGVIAQLPSTVTTFNQTGLKPGEEYTVTVV.ALKDQARSPPASDSIST
TNCchk .YLPAPEGLKFKSVRETSVQVEWDPLSISFDGWELVFRNMQKKDDNGDITSSLKRPETSYMQPGLAPGQQYNVSLH.IVKNNTRGPGLSRVITT
TNChum .YLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNK.EDEGEITKSLRRPETSYRQTGLAPGQEYEISLH.IVKNNTRGPGLKRVTTT
m .YLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNK.EDEGEITKSLRRPETSYRQTGLAPGQEYEISLH.IVKNNTRGPGLKKVTTT
p .YLPTPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNK.EDEGEITKSLRRPETTYRQTGLAPGQEYEISLH.IVKNNTRGPGLKRVTTT
n .HLPTTDDLRFKSVKETSVEVEWDPLDISFDTWDLIIRNTK..EENGEISTSLQRPVTSYVQTGLAPGETYNFSIH.VVKNSTRGPGLAKVTTT
HxB FN-III domain #3
TNRhum .VIDGPTQILVRDVSDTVAFVEWIPPRAKVDFILLKYGLVGGEGGRTTFRLQP..PLSQYSVQALRPGSRYEVSVSAVRGTNESDSATTQFTT
TNRrat VIDGPTQILVRDVSDTVAFVEWTPPRAKVDFILLKYGLVGGEGGKTTFRLQP..PLSQYSVQALRPGSRYEVSISAVRGTNESDASSTQFTT
TNRchk .LIDGPTQILVRDVSDTVAFVEWTPPRARVDAILLKYGLADGEGGRTTFRLQP..PLSQYSLQALRPGARYHLAVSALRGANESQPALAQFTT
TNCchk .KLDAPSQIEAKDVTDTTALITWSKPLAEIEGIELTYGPKDVPGDRTTIDLSE..DENQYSIGNLRPHTEYEVTLISRRGDMESDPAKEVFVT
TNChum .RLDAPSQIEVKDVTDTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTE..DENQYSIGNLKPDTEYEVSLISRRGDMSSNPAKETFTT
m .RLDAPSHIEVKDVTDTTALITWFKPLAEIDSIELSYGIKDVPGDRTTIDLTH..EDNQYSIGNLRPDTEYEVSLISRRVDMASNPAKETFIT
p .RLDAPSQIEAKDVTDTTALITWFKPLAEIDGIELTYGIKDVPGDRTTIDLTH..EENQYSIGNLKPDTEYEVSLISRRADMSSNPAKETFTT
n .RLDAPSQVEVRDVTDSMALVTWFRPLAQIDGVILSYGTGSQP..PTVVELSE..DESQYSLGNLIPDTEYEVTLLSRRGLMTSDPVTETFTT
HxB FN-III domain #4
TNRhum .EIDAPKNLRVGSRTATSLDLEWDNSEAEVQEYKVVYSTLAGEQYHEVLVPKGIGPTTRATLTDLVPGTEYGVGISAVMNSQQSVPATMNART
TNRrat EIDAPKNLRVGSRTATSLDLEWDNSEAEAQEYKVVYSTLAGEQYHEVLVPKGIGPTTKTTLTDLVPGTEYGVGISAVMNSKQSIPATMNART
TNRchk EIDAPKNLRVGSRTPASLELTWDNSEAEAHSYRVVYSTLAGEHYHEVLVPRDTGPTTRATLADLVPGTEYGIGISAVMDSQQSVPATMNART
TNCchk .DLDAPRNLKRVSQTDNSITLEWKNSHANIDNYRIKFAPISGGDHTELTVPKGNQATTRATLTGLRPGTEYGIGVTAVRQDRESAPATINAGT
TNChum .GLDAPRNLRRVSQTDNSITLEWRNGKAAIDSYRIKYAPISGGDHAEVDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKEDKESNPATINAAT
m .GLDAPRNLRRVSQTDNSITLEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKGDKESDPATINAAT
p .GLDAPRNLRRISQTDNSITLEWRNGKAAADTYRIKYAPISGGDHAEVEVPRSPQTTTKATLTGLRPGTEYGIGVSAVKGDKESDPATINAAT
n .DLDAPKNLRRVSQTDTTITLEWKNSQANVDLYRIKFAPLSTGDHAEITVPKSNQVTTKVTLTDLKPGTEYGIGVTAVKQDRESGPATINAAT
?? octopus? DNWITLEWKNSRSSIDGYRIKYGPIKGGAHGEDMFPKRAGDTTWATITGLRPGTEY
?? fish? DNSITLEWKNSRANVLNYRVKYGPLSGGEHGELVFPSGPQDTTQAKITGLAPGTEY
HxB FN-III domain #5
TNRhum ,ELDSPRDLMVTASSETSISLIWTKASGPIDHYRITFTPSSGIASEVTVPKDR....TSYTLTDLEPGAEYIISVTAERGRQQSLESTVDAFT
TNRrat ELDSPRDLMVTASSETSISLIWTKASGPIDHYRITFTPSSGISSEVTVPRDR....TSYTLTDLEPGAEYIISITAERGRQQSLESTVDAFT
TNRchk ELDSPRDLLVTASTETSISLSWTKAMGPIDHYRVTFTPASGMASEVTVSRNE....SQLTLSELEPGTEYTISIIAERGRQQSLEATVDAFT
TNCchk .DLDNPKDLEVSDPTETTLSLRWRRPVAKFDRYRLTYVSPSGKKNEMEIPVDS....TSFILRGLDAGTEYTISLVAEKGRHKSKPTTIKGST
TNChum .ELDTPKDLQVSETAETSLTLLWKTPLAKFDRYRLNYSLPTGQWVGVQLPRNT....TSYVLRGLEPGQEYNVLLTAEKGRHKSKPARVKAST
m .EIDAPKDLRVSETTQDSLTFFWTTPLAKFDRYRLNSSLPTGHSMEVQLPKDA....TSHVLTDLEPGQEYTVLLIAEKGRHKSKPARVKAST
p .DLDPPKDFRVSELKESSLTLLWRTPLAKFDRYRLNYGLPSGQPVEVQLPRNA....TSYILRGLEPGQEYTILLTAEKGRHKSKPARVKAST
n .DLDAPKDLQISGSSESTLSLRWKRPLAKFERYLISYISNTGKKNEIEVPGNV....NSFVLTGLDAGTEYSIAIVAEKGRHKSKPASVIGST
zFish IPGAA....NTYILTGLNPGMLHTITLTAERGRKMSAPATLSAST
_____________________________________________
Alternatively spliced domains
TN-C FN-III domain Q ZebraFish UNIQUE!
zFish DEEKPQVGNITISDVSWDSFSMSWDLDRGEVEGFLIEVSDPDGLSDGQNHTLSGQEF..SLAVTDLSPSTFYRVTLYGLYKGELLDPVFAEAIT
TNR alt splice domain R1 doesn't match anything
TNRhum .GFRPISHLHFSHVTSSSVNITWSDPSPPADRLILNYSPRDEEEEMMEVSLDATKR..HAVLMGLQPATEYIVNLVAVHGTVTSEPIVGSITT
TNRrat .GFRPISHLHFSHVTSSSVNITWSDPSPPADRLILNYSPRDEEEEMMEVLLDATKR..HAVLMGLQPATEYIVNLVAVHGTVTSEPIVGSITT
TNRchk .GVRPITQLHFSQLTSSSVNITWSDPSPPADRLVLTYSPRDEEAP.QQLALDGTRR..HASLTGLRPSTEYLVSLVAVHGAVSSEPVTGSITT
HxB FN-III domain A1
TNCchk EEEPELGNLSVSETGWDGFQLTWTAADGAYENFVIQVQQSDNPEETWNITVPGGQH..SVNVTGLKANTPYNVTLYGVIRGYRTKPLYVETTT
TNChum EQAPELENLTVTEVGWDGLRLNWTAADQAYEHFIIQVQEANKVEAARNLTVPGSLR..AVDIPGLKAATPYTVSIYGVIQGYRTPVLSAEAST
m EEVPSLENLTVTEAGWDGLRLNWTADDLAYEYFVIQVQEANNVETAHNFTVPGNLR..AADIPGLKVATSYRVSIYGVARGYRTPVLSAETST
HxB FN-III domain A2
TNChum GETPNLGEVVVAEVGWDALKLNWTAPEGAYEYFFIQVQEADTVEAAQNLTVPGGLR..STDLPGLKAATHYTITIRGVTQDFSTTPLSVEVLT
m GTTPNLGEVTVAEVGWDALTLNWTAPEGAYKNFFIQVLEADTTQTVQNLTVPGGLR..SVDLPGLKAATRYYITLRGVTQDFGTAPLSVEVLT
HxB FN-III domain A3
TNChum EEVPDMGNLTVTEVSWDALRLNWTTPDGTYDQFTIQVQEADQVEEAHNLTVPGSLR..SMEIPGLRAGTPYTVTLHGEVRGHSTRPLAVEVVT
HxB FN-III domain A4
TNChum EDLPQLGDLAVSEVGWDGLRLNWTAADNAYEHFVIQVQEVNKVEAAQNLTLPGSLR..AVDIPGLEAATPYRVSIYGVIRGYRTPVLSAEAST
m EDLPQLGGLSVTEVSWDGLTLNWTTDDLAYKHFVVQVQEANNVEAAQNLTVPSSLR..AVDIPGLKADTPYRVSIYGVIQGYRTPMLSTDVST
r9 SEIPELEGLTVTEVSWDSLTLNWTTDDLAYKHFIIQVQEANNVEAARNLTVSGSLR..VVDIPGLKADTPYTVSIYGVIQGYRTPMLSADVST
Only 12 mismatches between mouse and rat; 27 mismatches (63 identities) with human
HxB FN-III domain B
TNCchk GAHPEVGELTVSDITPESFNLSWTTTNGDFDAFTIEIIDSNRLLEPMEFNISGNSR..TAHISGLSPSTDFIVYLYGISHGFRTQAISAAATT
TNChum AKEPEIGNLNVSDITPESFNLSWMATDGIFETFTIEIIDSNRLLETVEYNISGAER..TAHISGLPPSTDFIVYLSGLAPSIRTKTISATATT
m AREPEIGNLNVSDVTPKSFNLSWTATDGIFDMFTIEIIDSNRLLQTAEHNISGAER..TAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATT
r RKEPEIGNLNISDVTPESFNLSWTATDRIFDMFTIEIIDSNRLLQTAEHNISGAER..TAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATT
p AGEPEIGNLSVSDITPESFSLSWTATEGAFETFTIEIIDSNRFLETMEYNISGAER..TAHISGLRPGNDFIVYLSGLAPGIQTKPISATATT
HxB FN-III domain AD2
TNCchk VEEPLLSKLTVSNATSDSMLLMWEAQDNAFDHFILEVRNSDSPLDSLVQIVPGASR..HYVVTNLKAATNYTVQLHGVVDGQGGQTLTALATT
HxB FN-III domain AD1
TNChum EPKPQLGMLIFSNITPKSFNMSWTTQAGLFAKIVINVSDAHSLHESQQFTVSGDAK..QAHITGLVENTGYDVSVAGTTLAGDPTRPLTAFVIT
TNCchk EAEPQLGTLTLTNVTPDSFNLSWTTRDGPFAKFVIHVRDSFAAHEPQELTVSGGAR..SAHISGLLDYTGYDINIKGTTDAGVHTEPLTAFVMT
HxB FN-III domain C
TNChum EALPLLENLTISDINPYGFTVSWMASENAFDSFLVTVVDSGKLLDPQEFTLSGTQR..KLELRGLITGIGYEVMVSGFTQGHQTKPLRAEIVT
TNCchk EAMPPLENLTVSDINPYGFTVSWMASENAFDSFLVVVVDSGKLLDPQEFLLTGAQR..QLKLKGLITGIGYEVMLYGFAKGHQTKPLSTVAVT
HxB FN-III domain D
TNCchk EAEPEVDNLLVSDATPDGFRLSWTADDGVFDSFVLKIRDTKRKSDPLELIVPGHER..THDITGLKEGTEYEIELYGVSSGRRSQPINSVATT
TNChum EAEPEVDNLLVSDATPDGFRLSWTADEGVFDNFVLKIRDTKKQSEPLEITLLAPER..TRDITGLREATEYEIELYGISKGRRSQTVSAIATT
m EAEPEVDNLLVSDATPDGFRLSWTADEGIFDSFVIRIRDTKKQSEPQEISLPSPER..TRDITGLREATEYEIELYGISRGRRSQPVSAIATT
r EAEPEVDNLLVSDATPDGFCLSWTADEGIFDSFVIRIRDTKKQSEPQEITLPSPDR..TRDITGLREATEYEIELYGISRGRRSQPVSAIATT
p EAEPEVDNLLVSDATPDGFRLSWTADEGVFDSFVLKIRDTKKQSEPLEITLLASER..TRDITGLREATEYEIELYGISSGKRSQPVSAIATT
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Last three invariant FN-III domains
Human HxB FN-III domain #6
TNRhum .GIDPPKDITISNVTKDSVMVSWSPPVASFDYYRVSYRPTQVGRLDSSVVPNTVTEF...TITRLNPATEYEISLNSVRGREESERICTLVHT
TNRrat .GIDPPKNITISNVTKDSLTVSWSPPVAPFDYYEYPIDHPS.GRLDSSVVPNTVTEF...TITRLYPASQYEISLNSVRGREESERICTLVHT
TNRchk .GMDAPKDLRVGNITQDSMVIYWSPPVAPFDHYRISYRAAE.GRTDSTAIGNDATEY...IMRLLQPATKYEIGVKSVRGREESEVASITTYT
TNXhum PVLESPRDLQFSEIRETSAKVNWMPPPSRADSFKVSYQLADGGEPQSVQVDGQARTQK...LQGLIPGARYEVTVVSVRGFEESEPLTGFLTT
TNXmus PVLESPRDLQFSDIGETSAKVKWVPPTSRVDSFKISYQLADGGEPQSVQVDGRTQTQI...LQGLIPDTRYEVTVVSVRGFEESEPLTGFLTT
TNXpig MRGFEESEPLTGFLTT
TNCchk .VVGSPKGISFSDITENSATVSWTPPRSRVDSYRVSYVPITGGTPNVVTVDGSK.TRT..KLVKLVPGVDYNVNIISVKGFEESEPISGILKT
TNChum .AMGSPKEVIFSDITENSATVSWRAPTAQVESFRITYVPITGGTPSMVTVDGTK.TQT..RLVKLIPGVEYLVSIIAMKGFEESEPVSGSFTT
m .AMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPMTGGAPSMVTVDGTD.TET..RLVKLTPGVEYRVSVIAMKGFEESDPVSGTLIT
r .AMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPVTGGPPSMVTVDGTD.TET..RLVRLTPGVEYHVSVIAMKGFEESDPVSGSLIT
p .AMGSPKEITFSDITENSATVSWMVPTAQVESFRITYVPITGGAPSVVTVDGTK.TQT..RLLRLLPGVEYLVSVIAVKGFEESEPVSGTLTT
n .AVGAPKGLSFSDITENSATVSWSAPQTRVDSFKVTYVPASGGVPQTVTVDGTK.TRT..TLVKLTPGVEYIVTVVSVKALDESGPISGPLTT
zFish .GLGAPKGIRFSDVTDTSATVHWTMPHTRVDNYRVIYVPIQGGSPLTLRVDGGE.SQA..MLSNLTPGVTYQVTVIAVKGLEESEPGSERVTT
Human HxB FN-III domain #7
TNRhum .AMDNPVDLIATNITPTEALLQWKAPVGEVENYVIVLTHFAVAGETILVDGVSEE....FRLVDLLPSTHYTATMYATNGPLTSGTISTNFST
TNRrat AMDSPMDLIATNITPTEALLQWKAPMGEVENYVIVLTHFAMAGETILVDGVSEE....FQLVDLLPRTHYTVTMYATSGPLVSGTIATNFST
TNRchk .AMDAPLGVTATNITPTEALLQWNPPLMDVESYVLVLTR..HTGETILVDGINQE....YQLTNLQPSTTYTVAMYATNGPLTSQTISTNFTT
TNXhum .VPDGPTQLRALNLTEGFAVLHWKPPQNPVDTYDVQVTAPGAPPLQAETPGSAVD....YPLHDLVLHTNYTATVRGLRGPNLTSPASITFTT
TNXmus .VPDGPTQLRALNLTDGSALLHWKPPHKPVDKYDVEVESPGAPPLQASAPGSAVD....YPLTDLALDTNYTATVRGLRGPNFTSPASITFTT
TNXpig .VPDGPTQLRALNLTEGSALLHWKPPQTPVDTYDVKVTASGAPSLQGSAPGSAVD....YPLHGLELHTNYTATLRGLRGPNLTSPASITFTT
TNCchk .ALDSPSGLVVMNITDSEALATWQPAIAAVDNYIVSYSSEDEPEVTQMVSGNTVE....YDLNGLRPATEYTLRVHAVKDAQKSETLSTQFTT
TNChum .ALDGPSGLVTANITDSEALARWQPAIATVDSYVISYTGEKVPEITRTVSGNTVE....YALTDLEPATEYTLRIFAEKGPQKSSTITAKFTT
m .ALDGPSGLLIANITDSEALAMWQPAIATVDSYVISYTGERVPEVTRTVSGNTVE....YELHDLEPATEYILSIFAEKGQQKSSTIATKFTT
r .ALDGPSGLLTANITDSEALAMWQPAIATVDSYVISYTGERVPEITRTVSGNTVE....LELHDLEPATEYTLSVFAEKGHQKSSTTATKFTT
p .ALDGPSGLVTANITDSEALAMWQPAIAPVDHYVISYTGDRVPEITRTVSGNTVE....YALTNLEPATEYTLRIFAEKGPQKSSTITTKFTT
n .ALDSPSGLKAVNVTETEAIALWQPSIASVDNYVLSYAADNDPETTKTISGNNVE....SDITGLQPSTQYTLTIYAVRGPQKSATMSTKFTT
TNCzfsh .ALDKPRGLTAVNISDTEALLLWQPSIATVDGYVITYSADSVAPVMERVSGNTVE....FEMSSLTPATLYTVKVYAFRDTAKSAATSTDFTT
Human HxB FN-III domain #8
TNRhum .HLDPPANLTASEVTRQSALISWQPPRAEIENYVLTYKSTDGSRKELIVDAED....TWIRLEGLLENTDYTVLLQAAQDTTWSSITSTAFTT
TNRrat .LLDPPANLTASEVTRQSALISWQPPRAAIENYVLTYKSTDGSRKELIVDAED....TWIRLEGLSENTDYTVLLQAAQEATRSSLTSTIFTT
TNRchk .LLDPPTNLTASEVTRRSALLSWVPPVGDIENYILTYRSTDGSRKELIVDAED....TWIRLEGLSETTQYTVRLQAAQNAMRSGFISTTFTT
TNXhum .GLEAPRDLEAKEVTPRTALLTWTEPPVRPAGYLLSFHTPGGQNQEILLPGGI....TSHQLLGLFGSTSYNARLQAMWGQSLLPPVSTSFTT
TNXmus .GLKPPQDLEAKEVTPRTALLTWTEPEVPPTGYLLSFDTPGGQIQEILLPAGT....TSHRLLRLFPSTFYSAQLRAIWGESLTPPVLTSFTT
TNXpig .GLEAPQDLEAKEVTPRTVLLTWTAPQVPPTGYLITFNTPGGQTQEILLPGGV....TSHRLQGLFPSTPYSAWLRAMWGESFTPPVSTSFTT
TNYchk ..GAPGTLWVGTLWPRSAHLHWAPPHVPPEGYNLIYGPPGGPVKTLQLPPEA....TSKELWGLEPSGRYRVQL...WGRGL.EPLETTFDT
TNCchk .GLDAPKDLSATEVQSETAVITWRPPRAPVTDYLLTYESIDGRVKEVILDPET....TSYTLTELSPSTQYTVKLQALSRSMRSKMIQTVFTT
TNChum .DLDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDT....TSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTT
m .DLDSPREFTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDT....TSYSLADLSPSTHYSARIQALSGSLRSKLIQTIFTT
r .DLDSPRELTATEVQSETAFLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDT....TSYSLADLSPSTHYTARIQALSGSLRSKLIQTIFTT
p .DLDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTLKEVVVGPET....TSYSLSGLSPSTHYTARIQALNGPLRSKMSQTVFTT
n .ALDAPRDLAASEIQSETALLTWRPPRSIITGFILIYEAVDGTLKEVILGPDM....TSYNLVDLSPSTQYTVRLLAMNGDVRSKTIQTVFTT
TNCzfsh .DVDAPQNLAASNIQTETAMLTWKPPRADISGYILSFESADGVVKEVVLSPTA....TFYSMSQLTASTEYTVKLQAIAGPKRSRVISTVFLT
Hexabrachion terminal knob &endash; fibrinogen-like domain
Short linker (according to Doolittle this is really part of the fibrinogen domain)
TNRhum GGRVFPHP
TNRrat GGRVFSHP
TNRchk GGRVFANP
TNXhum GGLRIPFP
TNXmus GGQRIPFP
TNXpig GGLRIPFP
TNYchk PPLPHPHP
TNCchk TGLLYPYP
TNChum IGLLYPFP
TNCmus IGLLYPFP
TNCrat IGLLYPFP
TNCpig IGLLYPFP
TNCnwt TGLLYPFP
TNCzfsh IGVLYKHP
TNRhum QDCAQHLMNGDTLSGVYPIFLNGELSQKLQVYCDMTTDGGGWIVFQRRQNGQTDFFRKWADYRVGFGNVEDEFW
TNRrat QDCAQHLMNGDTLSGVYTIFLNGELSHKLQVYCDMTTDGGGWIVFQRRQNGQTDFFRKWADYRVGFGNLEDEFW
TNRchk QDCAQHLMNGDTLSGVYTISINGDLSQRVQVFCDMSTDGGGWIVFQRRQNGLTDFFRKWADYRVGFGNLEDEFW
TNXhum RDCGEEMQNGAGASRTSTIFLNGNRERPLNVFCDMETDGGGWLVFQRRMDGQTDFWRDWEDYAHGFGNISGEFW
TNXmus RDCGEELKNGPSASKTTTIFLNGNRERPLDVFCDMETDGGGWLVFQRRMDGQTDFWRDWEEYAHGFGNISGEFW
TNXpig RDCGEEMQNGVSTSRTTTIFLNGNRERPLNVFCDMETDGGGWLVFQRRMDGKTDFWRDWEDYAHGFGNISGEFW
TNYchk RDCAEEQLNGPGPSREVLIFLGGDRQRPLHVFCDMESNGGGWLVFQRRMDGGTDFWRGWEEYVHGFGNVSGEFW
TNCchk KDCSQALLNGEVTSGLYTIYLNGDRTQPLQVFCDMAEDGGGWIVFLRRQNGKEDFYRNWKNYVAGFGDPKDEFW
TNChum KDCSQAMLNGDTTSGLYTIYLNGDKAQALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFW
TNCmus RDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFW
TNCrat RDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYATGFGDRRE
TNCpig RDCSQAMLNGDTTSGLYTIYVNNDKAQKLEVFCDMTSDSGGWIVFLRRKNGREDFYRNWKAYAAGFGDLKEEFW
TNCnwt KDCSQALLNGETASGLYTIYLNGDKAKPQEEYCDMSEYGGGWIVFLRRVDGKEDFYRNWKTYTAGFGDPTKEFF
TNCzfish KDCSQALLNGDTTSGLYTIYLRGDESQPLQVYCDMTTDGGGWIVFVRRQSGKVEFFRNWKNYTAGFGDLNDEFW
TNRhum LGLDNIHRITSQGRYELRVDMRDGQEAAFASYDRFSVEDSRNLYKLRIGSYNGTAGDSLSYHQGRPFSTEDRDNDVAV
TNRrat LGLDNYHRITAQGRYELRVDMRDGQEAVFAYYDKFAVEDSRSLYKLRIGGYNGTAGDSLSYHQGRPFSTEDRDNDVAV
TNRchk LGLDNIHKITSQGRYELRIDMRDGQEAAYAYYDKFSVGDSRSLYKLRIGDYNGTSGDSLTYHQGRPFSTKDRDNDVAV
TNXhum LGNEALHSLTQAGDYSIRVDLRAGDEAVFAQYDSFHVDSAAEYYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNSLL
TNXmus LGNEALHSLTQAGDYSLRVDLRAGKEAVFAQYDFFRVDSAKENYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNNLL
TNXpig LGNEALHSLTKAGDYSLRVDLRAGEEAVFAQYESFQVDSAAEHYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNNLL
TNYchk LGNAALHTLTASGPTELRVDLRTPSDSAFARYRDFAVSGPEDNFRLHLGAYSGTAGDALSYHAGSPFSTRDHDPRGRP
TNCchk IGLENLHKISSQGQYELRVDLRDRGETAYAVYDKFSVGDAKTRYRLRVDGYSGTAGDSMTYHNGRSFSTFDKDNDSAI
TNChum LGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAI
TNCmus LGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAI
TNCpig LGLDALSKITAQGQYELRVDLRDHGETAYAVYDRFSVGDARTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAI
TNCnwt LGLENLHQITSQGQYELRVDLRDGAETAYAVYDKFFVGDSKTAYRLKVDGYSGTAGDSMTYHSGALFSTFDKDNDSAI
TNCzfish LGLSNLHKITSFGQYELRVDLRDKGESAYAQYDKFSISEPRARYKVHVGGYSGTAGDSMTYHHGRPFSTYDNDNDIAV
TNRhum TNCAMSYKGAWWYKNCHRTNLNGKYGESRHSQGINWYHWKGHEFSIPFVEMKMRPYNHRLMAGRKRQSLQF
TNRrat TNCAMSYKGAWWYKNCHRTNLNGKYGESRHSQGINWYHWKGHEFSIPFVEMKMRPYIHRLTAGRKRRALKF
TNRchk TNCAMSYKGAWWYKNCHRTNLNGKYGESRHSQGINWYHWKGHEFSIPFVEMKMRPYNHRNISGRKRRSLQL
TNXhum ISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRNFRSPAGGG
TNXmus ISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRNFQVPTRGT
TNYchk RPCAVAYTGAWWYRNCHYANLNGRYGVPYDHQGINWYPWKGFEYSIPFTEMKLRPQRD
TNXpig ISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYYWKGFEFSVPFTEMKLRPRSFRPPGRGG
TNCchk TNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSSFRNLEGRRKRA
TNChum TNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA
TNCpig TNCALSYKGAFWYKNCHRVNLMGRYGDNSHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA
TNCnwt TNCALSYKGAFWYKNCHRVNLMGRYGDNSHSQGVNWFHWKGHEYSIQFAEMKVRPVSFRNLEGRRRRA
TNCzfish TNCALSYKGAFWYKNCHRVNIMGRYGDNSHSKGVNWFHWKGHEHSVEFAEMKIRPANFRNFEGRKKRS