Kinesin Methods

Construction of Plasmids
  • Mutants, Chimeras and Truncated Motors.

    • Expression and Purification of Kinesin Motors
  • Bacterial Expression of Motors
  • Purification of Ncd Motor Domain Protein
  • Purification of Kinesin-1 Motor Domain Protein
  • Purification of Kar3 Motor Domain Protein

    • ATPase Assays
  • Steady-State ATPase Assays
  • Single Turnover ATPase Assays
  • Nucleotide Binding/Hydrolysis Assays
  • Assays with 32P-ATP

    • Microtubule Binding Assays
  • Pelleting Assay

    • Motility Assays
  • Motility using VE-DIC Microscopy
  • Motility with Fluorescent MTs
  • Motor Polarity using Fluorescent MTs
  • Motor Polarity with Axoneme-MTs
  • Single Motor Assays
  • Landing Assay

    • Reagents
  • Tubulin Preparation
  • Microtubule Assembly
  • Axoneme Preparation
  • Axoneme-microtubule Preparation

    • GPP As A Tag
  • Single Motor Assay
  • In Vivo Studies

    • Crystallization
  • Crystallization Trials
  • Motor Crystalization Conditions


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    Copyright 1996-2005. All rights reserved.

    Created 2 March 1999 21:00 GMT
    Modified 25 January 2005 8:11 GMT