Poss Lab

Education

1992   B.A. , Department of Biology, Carleton College (Northfield, MN)

1998   Ph.D. , Department of Biology, Massachusetts Institute of Technology (Cambridge, MA)

Research and Professional Experience

1991-1992 Undergraduate research: Laboratory of John L. Tymoczko, Carleton College. “Biochemical Characterization of the Interaction of In Vitro Translated Human D Protein with U1 snRNA”

1993-1997 Doctoral research: Laboratory of Susumu Tonegawa, Massachusetts Institute of Technology. “Examination of Diverse Physiological Functions of Mammalian Heme Oxygenases by Gene Targeting”

1998-2003 Postdoctoral research: Laboratory of Mark T. Keating, Children's Hospital and Harvard Medical School, Boston, MA. “Modeling Disease and Regeneration in Zebrafish”

2003-2009 Assistant Professor, Department of Cell Biology, Duke University

2009- Associate Professor with tenure, Department of Cell Biology, Duke University

 

Postgraduate honors and awards

1998 Development Traveling Fellowship

1999-2002 Helen Hay Whitney Foundation Fellowship

2003- Whitehead Scholar Award, Duke University Medical Center

2004- Scientist Development Grant, American Heart Association

2005- Basil O'Connor Award, March of Dimes Birth Defects Foundation

2006- Pew Scholar Award, The Pew Charitable Trusts

2007- Established Investigator Award, American Heart Association

2009- Early Career Scientist, Howard Hughes Medical Institute

 

Publications

Original Reports

1. Poss, K. D. , Thomas, M. J., Ebralidze, A. K., O'Dell, T. J., and Tonegawa, S. (1995). Hippocampal long-term potentiation is normal in Heme Oxygenase-2 mutant mice. Neuron 15, 867-873.

2. Poss, K. D. , and Tonegawa, S. (1997). Reduced stress defense in Heme Oxygenase-1-deficient cells. Proceedings of the National Academy of Sciences, USA 94, 10925-10930.

3. Poss, K. D. , and Tonegawa, S. (1997). Heme Oxygenase-1 is required for mammalian iron reutilization. Proceedings of the National Academy of Sciences, USA 94, 10919-10924.

4. Zakhary, R., Poss, K. D. , Jaffrey, S. R., Ferris, C. D., Tonegawa, S., and Snyder, S. H. (1997). Targeted gene deletion of Heme Oxygenase-2 reveals neural role for carbon monoxide. Proceedings of the National Academy of Sciences, USA 94, 14848-14853.

5. Burnett, A. L., Johns, D. G., Kriegsfeld, L. J., Klein, S. L., Calvin, D. C., Demas, G. E., Schramm, L. P., Tonegawa, S., Nelson, R. J., Snyder, S. H., and Poss, K. D. (1998). Ejaculatory abnormalities in mice with targeted disruption of the gene for Heme Oxygenase-2. Nature Medicine 4, 84-87.

6. Dennery, P. A, Spitz, D. R., Yang, G., Tatarov, A., Lee, C. S., Shegog, M. L., and Poss, K. D. (1998). Oxygen toxicity and iron accumulation in the lungs of mice lacking Heme Oxygenase-2. Journal of Clinical Investigation 101, 1001-1011.

7. Soares, M., Lin, Y., Anrather, J., Csizmadia, E., Takigami, K., Sato, K., Grey, S. T., Colvin, R. B., Choi, A. M., Poss, K. D. , and Bach, F. H. (1998). Expression of Heme Oxygenase-1 can determine cardiac xenograft survival. Nature Medicine 4, 1073-1077.

8. Ferris C. D., Jaffrey, S. R., Sawa, A., Takahashi, M., Brady, S. D., Barrow, R.K., Tysoe, S. A., Wolosker, H., Baranano, D. E., Dore, S., Poss, K. D. , and Snyder, S. H. (1999). Haem Oxygenase-1 prevents cell death by regulating cellular iron. Nature Cell Biology 1, 152-157.

9. Demas, G. E., Kriegsfeld, L. J., Poss, K. D. , Tonegawa, S., and Nelson, R. J. (1999). Circadian locomotor rhythms in mice with targeted disruption of the gene for the carbon monoxide synthesizing enzyme, Heme Oxygenase-2. Biological Rhythm Research 30, 282-289.

10. Nath, K. A., Haggard, J. J., Croatt, A. J., Grande, J. P., Poss, K. D. , and Alam, J. (2000). The indispensability of Heme Oxygenase-1 in protecting against acute heme protein-induced toxicity in vivo. American Journal of Pathology 156, 1527-1535.

11. Shiraishi, F., Curtis, L. M., Truong, L., Poss, K. D. , Visner, G. A., Madsen, K., Nick, H. S., and Agarwal, A. (2000). Heme Oxygenase-1 gene ablation or expression modulates cisplatin-induced renal tubular apoptosis. American Journal of Physiology 278, F726–F736.

12. Poss, K. D. , Shen, J., and Keating, M. T. (2000). Induction of lef1 during zebrafish fin regeneration. Developmental Dynamics 219, 282-286.

13. Poss, K. D. , Shen, J., Nechiporuk, A., McMahon, G., Thisse, B., Thisse, C., and Keating, M. T. (2000). Roles for Fgf signaling during zebrafish fin regeneration. Developmental Biology 222, 347-358.

14. Poss, K. D. , Nechiporuk, A., Hillam, A. M., Johnson, S. L., and Keating, M. T. (2002). Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration. Development 129, 5141-5149.

- see also: Alfred, J. (2003). Regeneration swims into view. Nature Reviews Genetics 4, 6.

15. Poss, K. D. , Wilson, L. G., and Keating, M. T. Heart regeneration in zebrafish. (2002). Science 298, 2188-2190.

- see also: Scott, I. C. and Stainier, D. Y. R. (2002). Fishing out a new heart. Science 298, 2141-2142.

16. Nechiporuk, A., Poss, K. D. , Johnson, S. L., and Keating, M. T. (2003). Positional cloning of a temperature-sensitive mutant emmental reveals a role for Sly1 during cell proliferation in zebrafish fin regeneration. Developmental Biology 258, 291-306.

17. Gillette-Gerguson I., Ferguson, D. G., Poss, K. D. , and Moorman, S. J. (2003). Changes in gravitational force induce alterations in gene expression that can be monitored in the live, developing zebrafish heart. Advances in Space Research 32, 1641-1646.

18. Traver, D., Paw, B. H., Poss, K. D. , Penberthy, W. T., Lin, S., and Zon, L. I. (2003). Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nature Immunology 4, 1238-1246.

19. Poss, K. D. , Nechiporuk, A., Stringer, K. F., Lee, C., and Keating, M. T. (2004). Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1 . Genes and Development 18, 1527-1532.

20. Lee, Y., Grill, S., Sanchez, A., Murphy-Ryan, M., and Poss, K. D. (2005). Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration. Development 132, 5173-5183.

21. Lepilina, A., Coon, A. N., Kikuchi, K., Holdway, J. E., Roberts, R. W., Burns, C. G., and Poss, K. D. (2006). A dynamic epicardial injury response supports progenitor cell activity during zebrafish heart regeneration. Cell 127, 607-619.

- see also: Curado, S. and Stainier, D. Y. R. (2006). The heArt of regeneration. Cell 127, 462-464.

22. Nechiporuk, A., Linbo, T., Poss, K. D. , and Raible, D. W. (2007). Specification of epibranchial placodes in zebrafish. Development 134, 611-623.

23. Wills A.A., Holdway J.E., Major R.J., Poss K.D. (2008). Regulated addition of new myocardial and epicardial cells fosters homeostatic cardiac growth and maintenance in adult zebrafish. Development 135, 183-192.

24. Yin, V., Thompson, J. M., Thummel, R., Hyde, D., Hammond, S., and Poss, K. D. (2008). Fgf-dependent depletion of microRNA-133 promotes zebrafish appendage regeneration. Genes and Development 22, 728-733.

25. Wills, A.A., Kidd, A.R., Lepilina, A., and Poss, K. D. (2008). Fgfs control homeostatic regeneration in adult zebrafish fins. Development 135, 3063-3070.

26. Marques, S., Lee, Y., Poss, K. D. , and Yelon, D. (2008) Reiterative roles for FGF signaling in the establishment of size and proportion of the zebrafish heart. Developmental Biology 321, 397-406.

27. Waxman, J. S., Keegan, B. R., Roberts, R. W., Poss, K. D. , and Yelon, D. (2008). Hox5b acts downstream of retinoic acid signaling in the forelimb field to restrict heart field potential in zebrafish. Developmental Cell 15, 923-934.

28. Lee, Y., Hami, D., De Val, S., Kagermeier-Schenk, B., Wills, A. A., Black, B. L., Weidinger, G., and Poss, K. D. (2009). Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins. Developmental Biology 331, 270-280.

 

Invited Reviews

1. Poss, K. D. (2003). Zebrafish fin and heart regeneration. Cell Technology 23, 41-44.

2. Poss, K. D. , Keating, M. T., and Nechiporuk, A. (2003). Tales of regeneration in zebrafish. Developmental Dynamics 226, 202-210.

3. Poss, K. D. (2004). A zebrafish model of germ cell aneuploidy. Cell Cycle 3, 1225-1226.

4. Poss, K. D. (2007). Getting to the heart of regeneration in zebrafish. Seminars in Cell and Developmental Biology (in press)

5. Major, R. J. and Poss, K. D. (2007). Zebrafish heart regeneration as a model for cardiac tissue repair. Drug Discovery Today: Disease Models 4, 219-225.

6. Yin, V. and Poss, K. D. (2008). New regulators of vertebrate appendage regeneration. Current Opinion in Genetics and Development 18, 381-386.

7. Nachtrab, G. and Poss, K. D. (2009). Genetic DISC-section of regeneration in Drosophila. Developmental Cell 16, 777-778.

 

Book Chapters

Poss, K. D. (2009). Cardiac regeneration in the zebrafish model system. In “Heart Development and Regeneration”, edited by R. P. Harvey and N. Rosenthal, (Elsevier, Inc.)